What question did this study set out to answer?

To determine whether the mitogenic activity observed in certain LPS preparations on C3H/HeJ spleen cells is caused by lipid A itself or an associated contaminant.

September 1, 1976The Journal of Experimental MedicineOpen Access

Isolation of a lipid a bound polypeptide responsible for LPS- initiated mitogenesis of C3H/HeJ spleen cells

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Authors

DMDC MorrisonScripps Health SBSJ BetzScripps Health DJDM JacobsUniversity at Buffalo, State University of New York

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Implication

Laboratory study reveals a lipid A-associated polypeptide drives mitogenesis in C3H/HeJ mouse spleen cells, indicating this strain is genuinely unresponsive to pure lipid A.

Key Points

To determine whether the mitogenic activity observed in certain LPS preparations on C3H/HeJ spleen cells is caused by lipid A itself or an associated contaminant.
Extracted lipopolysaccharide (LPS) and lipid A preparations using aqueous butanol and hot-phenol extraction techniques.
Assessed B-lymphocyte mitogenic response in C3H/HeJ mouse spleen cells after complexing LPS preparations with polymyxin B.
Isolated and partially purified the active fraction using gel filtration chromatography and cesium chloride equilibrium density gradient centrifugation.
Identified that mitogenic activity in positive LPS preparations is caused by a low-molecular-weight, phenol-soluble polypeptide of approximately 10,000 Da.
Demonstrated that the partially purified polypeptide initiates significant mitogenesis in C3H/HeJ splenocytes at concentrations as low as 10 µg/ml.
Confirmed that hot-phenol extraction eliminates mitogenic activity in C3H/HeJ spleen cells by removing this polypeptide, establishing that C3H/HeJ mice are true nonresponders to lipid A.

Cite This Study

Morrison et al. (1976) studied this question.

synapsesocial.com/papers/6a7ccee221c5894b35c8a165 https://doi.org/10.1084/jem.144.3.840

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Implication

Laboratory study reveals a lipid A-associated polypeptide drives mitogenesis in C3H/HeJ mouse spleen cells, indicating this strain is genuinely unresponsive to pure lipid A.

Isolation of a lipid a bound polypeptide responsible for LPS- initiated mitogenesis of C3H/HeJ spleen cells

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Implication

Key Points

Cite This Study

Isolation of a lipid a bound polypeptide responsible for LPS- initiated mitogenesis of C3H/HeJ spleen cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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