January 28, 2010Molecular EndocrinologyOpen Access

Partially Deglycosylated Equine LH Preferentially Activates β-Arrestin-Dependent Signaling at the Follicle-Stimulating Hormone Receptor

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Population

HEK-293 cells stably expressing the FSH-R

Comparison

Deglycosylated equine LH [truncated eLHbeta… vs Native eLH

Design

Preclinical

Authors

VWVanessa L. WehbiUniversité de Tours TTThibaud TranchantInserm GDGuillaume DurandInstitut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Discussion

Member takes

Implication

Hypothesis-generating for beta-arrestin-biased FSH-R ligands in animals; leaves open translation to human physiology or therapy.

Structured PICO

Population

HEK-293 cells stably expressing the FSH-R

Intervention

Deglycosylated equine LH (eLHdg) [truncated eLHbeta (Delta121-149) combined with asparagine56-deglycosylated eLHalpha]

Comparator

Native eLH

Outcome

beta-arrestin-dependent signaling (cAMP accumulation, protein kinase A activity, cAMP-responsive element-dependent transcriptional activity, receptor internalization, beta-arrestin recruitment, ERK and rpS6 phosphorylation)surrogate

Deglycosylated equine LH acts as a biased agonist at the FSH receptor, preferentially activating beta-arrestin-dependent signaling over G-protein coupling.

Cite This Study

Wehbi et al. (2010) studied this question.

synapsesocial.com/papers/6a7ccfc5c576c1b0b4f46dfe https://doi.org/10.1210/me.2009-0347

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Partially Deglycosylated Equine LH Preferentially Activates β-Arrestin-Dependent Signaling at the Follicle-Stimulating Hormone Receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Partially Deglycosylated Equine LH Preferentially Activates β-Arrestin-Dependent Signaling at the Follicle-Stimulating Hormone Receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider