What question did this study set out to answer?

To determine how renin isoelectric heterogeneity influences renal renin storage, secretion pathways, and circulating renin composition under basal and stimulated conditions.

October 1, 1993Journal of the American Society of NephrologyOpen Access

Role of renin isoelectric heterogeneity in renal storage and secretion of renin.

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Population

in vivo blood perfused rabbit kidney preparation

Comparison

Acute stimulation of renin secretion vs Basal conditions

Design

Preclinical

Authors

JOJohn A. OpsahlAlberta Hospital Edmonton PAPaul A. AbrahamHospices Civils de Lyon JSJay G. ShakeSan Diego Cardiac Center

Discussion

Member takes

Implication

Reinforces two-pool renin secretion model in animals; leaves open whether isoelectric heterogeneity affects human outcomes or therapies.

Key Points

To determine how renin isoelectric heterogeneity influences renal renin storage, secretion pathways, and circulating renin composition under basal and stimulated conditions.
Evaluated an in vivo blood-perfused rabbit kidney model under basal and acutely stimulated states.
Induced acute secretory stimulation via reduced renal perfusion pressure combined with angiotensin-converting enzyme inhibition.
Directly measured and compared the isoelectric charge profiles of stored renal renin, newly secreted renin, and systemic circulating renin.
Under basal conditions, the kidney preferentially stored and secreted relatively basic isoelectric forms of renin over acidic forms.
Acute stimulation selectively drove the release of basic renin forms into the renal venous effluent with minimal effect on acidic forms.
Circulating renin became disproportionately enriched with basic forms after acute stimulation, producing an overall circulating renin pool characterized by a shorter half-life.

Structured PICO

Population

in vivo blood perfused rabbit kidney preparation

Intervention

Acute stimulation of renin secretion (reduced renal perfusion pressure and angiotensin-converting enzyme inhibition)

Comparator

Basal conditions

Outcome

Isoelectric profile of renal renin, secreted renin, and circulating reninsurrogate

Renin isoelectric heterogeneity reflects a two-pool model where acidic forms are constitutively secreted and basic forms are stored and rapidly released upon acute stimulation.

Cite This Study

Opsahl et al. (1993) studied this question.

synapsesocial.com/papers/6a7cd87ae9867978b6a1e25f https://doi.org/10.1681/asn.v441054

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Role of renin isoelectric heterogeneity in renal storage and secretion of renin.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Role of renin isoelectric heterogeneity in renal storage and secretion of renin.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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