Regional Alterations in the Expression of Smooth Muscle Myosin Isoforms in Response to Partial Bladder Outlet Obstruction

Anita Mannikarottu; Joseph Hypolite; Stephen A. Zderic; Alan J. Wein; Samuel Chacko; Michael E. DiSanto

doi:10.1097/01.ju.0000142100.06466.49

January 1, 2005The Journal of Urology

Regional Alterations in the Expression of Smooth Muscle Myosin Isoforms in Response to Partial Bladder Outlet Obstruction

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Key Points

To determine whether partial bladder outlet obstruction induces region-specific variations in smooth muscle myosin isoform expression and contractile function across the detrusor.
Obtained detrusor samples from 9 designated bladder regions in adult New Zealand White rabbits subjected to partial bladder outlet obstruction (defined as >30 voids per 24 hours) or sham surgery.
Assessed mRNA and protein expression of smooth muscle myosin isoforms (SM-A, SM-B, SMemb) using RT-PCR, SDS-PAGE, and Western blotting.
Evaluated tissue contractile responses across regions following stimulation with bethanechol and potassium chloride.
Sham-operated bladders showed uniform detrusor expression with nearly complete SM-B expression across all 9 regions.
Obstructed bladders displayed regional divergence: the dome expressed ~70% SM-B and 30% SM-A (with a ~2-fold higher SM-B protein level), whereas the base expressed ~35% SM-B and 65% SM-A.
SMemb isoform expression increased significantly at mRNA and protein levels exclusively in the dome region, correlating with localized changes in detrusor contractility.

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Implication

Controlled animal study demonstrates regional variation in smooth muscle myosin isoform shifts in obstructed rabbit bladders, indicating localized molecular drivers of altered detrusor contractility.

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Mannikarottu et al. (2005) studied this question.

synapsesocial.com/papers/6a7ceb9ba0d4e77c65aeb4bf https://doi.org/10.1097/01.ju.0000142100.06466.49

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Regional Alterations in the Expression of Smooth Muscle Myosin Isoforms in Response to Partial Bladder Outlet Obstruction

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Regional Alterations in the Expression of Smooth Muscle Myosin Isoforms in Response to Partial Bladder Outlet Obstruction

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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