What question did this study set out to answer?

To establish and validate a candidate Reference Measurement Procedure for the simultaneous quantification of serum free triiodothyronine (FT3) and free thyroxine (FT4) using equilibrium dialysis combined with isotope-dilution liquid chromatography-tandem mass spectrometry.

December 17, 2025Clinical Chemistry and Laboratory Medicine (CCLM)

Development and validation of a candidate reference measurement procedure for free triiodothyronine and free thyroxine in human serum using equilibrium dialysis isotope-dilution liquid chromatography-tandem mass spectrometry

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Authors

MZMin ZhanKorea University QZQiaoxuan ZhangGuangzhou University of Chinese Medicine ZMZijia MaThe First People’s Hospital of Lianyungang

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Implication

Methodological validation study demonstrates precise quantification of serum free thyroid hormones via mass spectrometry, highlighting a reference standard to harmonize clinical assays.

Key Points

To establish and validate a candidate Reference Measurement Procedure for the simultaneous quantification of serum free triiodothyronine (FT3) and free thyroxine (FT4) using equilibrium dialysis combined with isotope-dilution liquid chromatography-tandem mass spectrometry.
Employed an optimized, reusable equilibrium dialysis device to separate unbound FT3 and FT4 from serum protein-bound fractions under controlled conditions of temperature, pH, membrane type, and duration.
Quantified FT3 and FT4 directly in the dialysate using isotope-dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS), accompanied by cross-method comparisons and uncertainty evaluations.
Achieved a limit of quantification of 1.54 pmol/L for FT3 and 3.22 pmol/L for FT4, with an analytical imprecision of less than 3% and zero detected interference from endogenous analogs.
Demonstrated robust consistency across laboratory comparisons, whereas routine chemiluminescent immunoassays displayed poor agreement with the candidate reference procedure.

Cite This Study

Zhan et al. (2025) studied this question.

synapsesocial.com/papers/6a7ceca1d925dff0595e0287 https://doi.org/10.1515/cclm-2025-0874

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Implication

Methodological validation study demonstrates precise quantification of serum free thyroid hormones via mass spectrometry, highlighting a reference standard to harmonize clinical assays.

Development and validation of a candidate reference measurement procedure for free triiodothyronine and free thyroxine in human serum using equilibrium dialysis isotope-dilution liquid chromatography-tandem mass spectrometry

Discussion

Member takes

Implication

Key Points

Cite This Study

Development and validation of a candidate reference measurement procedure for free triiodothyronine and free thyroxine in human serum using equilibrium dialysis isotope-dilution liquid chromatography-tandem mass spectrometry

Discussion

Member takes

Implication

Key Points

Cite This Study

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