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February 6, 2001Proceedings of the National Academy of SciencesOpen Access

A zinc finger-containing papain-like protease couples subgenomic mRNA synthesis to genome translation in a positive-stranded RNA virus

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Population

nidovirus equine arteritis virus model

Design

Preclinical

Authors

MTMarieke A. TijmsSolvay (Netherlands)LDLeonie C. van DintenLeiden University Medical CenterAGAlexander E. GorbalenyaUniversity of Southern California

Discussion

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Overview

May enable selective antiviral targeting of nidovirus transcription; leaves open relevance to human coronaviruses.

Structured PICO

P
Population
nidovirus equine arteritis virus model
I
Intervention
site-directed mutagenesis of the predicted N-terminal zinc finger of nonstructural protein 1 (nsp1)
O
Outcome
viral replication and subgenomic mRNA synthesis (transcription)

Demonstrates that the nsp1 protein in equine arteritis virus couples replicase expression and subgenomic mRNA synthesis, with its zinc finger domain being crucial for transcription.

Cite This Study

Tijms et al. (2001) studied this question.

synapsesocial.com/papers/6a7cf710e5dc50daa6ec23e5https://doi.org/10.1073/pnas.98.4.1889
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Also Consider

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