What question did this study set out to answer?

To identify the specific protein kinase C (PKC) isozymes that mediate the inhibition of chloride secretion and the reduction in transepithelial barrier resistance.

August 1, 2001AJP Cell Physiology

Regulation of epithelial transport and barrier function by distinct protein kinase C isoforms

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Authors

JSJaekyung C. SongRural Development Administration CHCelina M. HansonUppsala University VTVance TsaiSunnybrook Health Science Centre

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Implication

In vitro study reveals that distinct protein kinase C isoforms differentially regulate epithelial chloride transport and barrier integrity in T84 cells, indicating pathway-specific targets.

Key Points

To identify the specific protein kinase C (PKC) isozymes that mediate the inhibition of chloride secretion and the reduction in transepithelial barrier resistance.
Exposed T84 epithelial cell monolayers to PKC activators (PMA, bryostatin-1, and carbachol) alone or in the presence of selective PKC inhibitors (Gö-6850, Gö-6976, and rottlerin).
Monitored chloride secretion via short-circuit current (Isc) and epithelial barrier integrity via transepithelial electrical resistance (TER) using voltage-current clamp technique.
Characterized PKC isoform activation, membrane translocation, and subcellular localization using cell fractionation, Western blotting, kinase assays, and immunofluorescence.
Basolateral translocation and activation of novel PKC epsilon mediated the inhibition of cAMP-stimulated chloride secretion, an effect blocked by Gö-6850 but not by Gö-6976 or rottlerin.
Apical translocation of conventional PKC alpha mediated the reduction in transepithelial resistance, which was prevented by both Gö-6850 and Gö-6976.
Carbachol selectively translocated PKC epsilon and inhibited chloride secretion without impairing barrier resistance, whereas bryostatin-1 translocated both isoforms but caused only transient resistance decreases due to PKC alpha downregulation.

Cite This Study

Song et al. (2001) studied this question.

synapsesocial.com/papers/6a7cfb45a3cb31c5f99295ac https://doi.org/10.1152/ajpcell.2001.281.2.c649

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Implication

In vitro study reveals that distinct protein kinase C isoforms differentially regulate epithelial chloride transport and barrier integrity in T84 cells, indicating pathway-specific targets.

Regulation of epithelial transport and barrier function by distinct protein kinase C isoforms

Discussion

Member takes

Implication

Key Points

Cite This Study

Regulation of epithelial transport and barrier function by distinct protein kinase C isoforms

Discussion

Member takes

Implication

Key Points

Cite This Study

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