Lactobacillus gasseri BNR17 Exhibits Superior Adhesion in a Human Intestinal Organoid-Derived Model

Adhesion to the human gut epithelium is essential for the beneficial effects of probiotics.A human organoid-derived intestinal epithelial cell (IEC) monolayer model recapitulates key features of the gut epithelium and overcomes limitations of three-dimensional cultures, providing a suitable platform for assessing bacterial adhesion.In this study, we evaluated the adhesion capacity of the probiotic strain Lactobacillus gasseri BNR17 to human IECs using fluorescence-based analysis and colony-forming unit assays.BNR17 retained high fluorescence intensity after washing and exhibited approximately 20-fold greater viable adhesion than the control strain Limosilactobacillus fermentum ACE21.These results demonstrate the strong adhesive capacity of L. gasseri BNR17, supporting its potential for effective gut colonization and health promotion.