What question did this study set out to answer?

To assess the capability of fluorescent amplified-fragment length polymorphism (FAFLP) analysis to identify and subtype epidemic methicillin-resistant Staphylococcus aureus phage type 15 (EMRSA-15).

October 1, 1999Journal of Clinical MicrobiologyOpen Access

Genotyping of Epidemic Methicillin-Resistant Staphylococcus aureus Phage Type 15 Isolates by Fluorescent Amplified-Fragment Length Polymorphism Analysis

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To assess the capability of fluorescent amplified-fragment length polymorphism (FAFLP) analysis to identify and subtype epidemic methicillin-resistant Staphylococcus aureus phage type 15 (EMRSA-15).
Digested bacterial DNA using ApaI/TaqI or EcoRI/MseI restriction enzymes, ligated site-specific adaptors, and amplified fragments via adaptor-specific PCR primers.
Separated fluorescent fragments on an automated sequencer, analyzed data with Genescan software, and performed UPGMA cluster analysis based on fragment presence or absence.
Compared FAFLP typing against coagulase gene PCR-restriction fragment length polymorphism (PCR-RFLP) and pulsed-field gel electrophoresis (PFGE).
FAFLP distinguished EMRSA-15 from other MRSA isolates and separated 24 EMRSA-15 isolates into 11 distinct profiles when combining both enzyme-primer data sets.
PCR-RFLP analysis of the coagulase gene failed to discriminate among the tested EMRSA-15 isolates.
PFGE discriminated between isolates but was less reproducible than FAFLP, which successfully typed all tested isolates.

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Implication

Comparative laboratory study demonstrates high discriminatory power and reproducibility of FAFLP in MRSA-15 isolates, highlighting its utility for hospital infection control.

Cite This Study

Grady et al. (1999) studied this question.

synapsesocial.com/papers/6a7d08922d005ba2c41cc4aa https://doi.org/10.1128/jcm.37.10.3198-3203.1999

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Implication

Comparative laboratory study demonstrates high discriminatory power and reproducibility of FAFLP in MRSA-15 isolates, highlighting its utility for hospital infection control.

Genotyping of Epidemic Methicillin-Resistant Staphylococcus aureus Phage Type 15 Isolates by Fluorescent Amplified-Fragment Length Polymorphism Analysis

Key Points

Discussion

Implication

Cite This Study

Genotyping of Epidemic Methicillin-Resistant Staphylococcus aureus Phage Type 15 Isolates by Fluorescent Amplified-Fragment Length Polymorphism Analysis

Key Points

Discussion

Implication

Cite This Study

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