A hepatocyte membrane-based culture system recapitulating the physiological microenvironment of hepatic stellate cells

This is the authors' abstract. We don't add key points for this paper.

We developed a hepatocyte plasma membrane-based culture system that mimics in vivo cell-cell adhesion to regulate hepatic stellate cell (HSC) phenotypes. This platform maintains HSC quiescence by reducing activation markers and preserving a quiescent morphology, while allowing the observation of HSC activation in response to TGF-β treatment. It provides a physiologically relevant tool for studying HSC regulation and screening antifibrotic therapies, advancing in vitro modeling of liver fibrosis.