What question did this study set out to answer?

To investigate how mutations in the Staphylococcus aureus virulence regulatory genes agr and sar influence bacterial autolysis and murein hydrolase activity.

July 15, 1998Journal of BacteriologyOpen Access

Opposing Roles of the Staphylococcus aureus Virulence Regulators, Agr and Sar, in Triton X-100- and Penicillin-Induced Autolysis

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Authors

DFDavid F. FujimotoSan Diego State University KBKenneth W. BaylesNebraska Medical Center

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Implication

In vitro genetic analysis reveals opposing regulation of autolysis by agr and sar in Staphylococcus aureus, suggesting coordinated control of murein hydrolase activity.

Key Points

To investigate how mutations in the Staphylococcus aureus virulence regulatory genes agr and sar influence bacterial autolysis and murein hydrolase activity.
Generated and evaluated Staphylococcus aureus strains harboring mutations within the virulence regulatory genes agr and sar.
Measured bacterial autolysis rates induced by Triton X-100 and penicillin.
Performed zymographic analyses on mutant strains to assess murein hydrolase expression and enzymatic activity profiles.
Mutations in agr resulted in decreased rates of bacterial autolysis.
Mutations in sar caused an increase in bacterial autolysis rates.
Zymographic analyses demonstrated that agr and sar modulate the expression and activity profiles of murein hydrolases.

Cite This Study

Fujimoto et al. (1998) studied this question.

synapsesocial.com/papers/6a7d0b7a00c5c9efbcaa0887 https://doi.org/10.1128/jb.180.14.3724-3726.1998

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Implication

In vitro genetic analysis reveals opposing regulation of autolysis by agr and sar in Staphylococcus aureus, suggesting coordinated control of murein hydrolase activity.

Opposing Roles of the Staphylococcus aureus Virulence Regulators, Agr and Sar, in Triton X-100- and Penicillin-Induced Autolysis

Discussion

Member takes

Implication

Key Points

Cite This Study

Opposing Roles of the Staphylococcus aureus Virulence Regulators, Agr and Sar, in Triton X-100- and Penicillin-Induced Autolysis

Discussion

Member takes

Implication

Key Points

Cite This Study

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