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May 1, 2002Journal of Biological ChemistryOpen Access

Alternate Translation Occurs within the Core Coding Region of the Hepatitis C Viral Genome

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Population

Hepatitis C virus isolates, in vitro translation models, Escherichia coli cells expressing recombinant…

Comparison

Assessment of core+1 ORF expression via… vs Positive control for luciferase activity

Design

Preclinical

Authors

AVAgoritsa VarakliotiLaiko General Hospital of AthensNVNiki VassilakiPasteur Hellenic InstituteUGUrania GeorgopoulouPasteur Hellenic Institute

Discussion

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Implication

Core+1 protein detection via frameshifting warrants further HCV model studies; leaves open any diagnostic or therapeutic role in humans.

Structured PICO

P
Population
Hepatitis C virus (HCV) isolates, in vitro translation models, Escherichia coli cells expressing recombinant core+1 protein, and HCV-positive sera
I
Intervention
Assessment of core+1 ORF expression via luciferase fusion, antisera against synthetic peptides, and site-directed mutagenesis
C
Comparator
Positive control (core-luciferase) for luciferase activity
O
Outcome
Evidence of core+1 ORF expression and translation mechanisms (+1/-1 ribosomal frameshift)surrogate

The study provides evidence for novel translation mechanisms (+1/-1 ribosomal frameshifting) within the core coding region of the HCV genome, demonstrating the expression of the core+1 ORF.

Cite This Study

Varaklioti et al. (2002) studied this question.

synapsesocial.com/papers/6a7d0d88166e996abdd59f2fhttps://doi.org/10.1074/jbc.m201722200
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Evidence for a new hepatitis C virus antigen encoded in an overlapping reading frame2001 · 256 citations
  2. 2Hepatitis C Virus Core from Two Different Genotypes Has an Oncogenic Potential but Is Not Sufficient for Transforming Primary Rat Embryo Fibroblasts in Cooperation with the H-<i>ras</i>Oncogene1998 · 137 citations
  3. 3Programmed translational frameshifting1996 · 314 citations
  4. 4Suppression of Host Immune Response by the Core Protein of Hepatitis C Virus: Possible Implications for Hepatitis C Virus Persistence1999 · 258 citations