What question did this study set out to answer?

To characterize the molecular mechanisms by which daptomycin disrupts bacterial cell wall biosynthesis and architecture in Enterococcus faecalis and Staphylococcus aureus.

July 28, 2023Scientific ReportsOpen Access

The effects of daptomycin on cell wall biosynthesis in Enterococcal faecalis

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Authors

BRBinayak RimalJohns Hopkins University JCJames D. ChangBeth Israel Deaconess Medical Center CLChengyin LiuChina Science and Technology Publishing & Media

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Implication

In vitro analysis demonstrates impaired peptidoglycan crosslinking in Enterococcus faecalis under daptomycin, indicating reduced maturation triggers autolytic wall degradation.

Key Points

To characterize the molecular mechanisms by which daptomycin disrupts bacterial cell wall biosynthesis and architecture in Enterococcus faecalis and Staphylococcus aureus.
Examined intact Staphylococcus aureus whole cells cultured with sub-inhibitory daptomycin in chemically defined medium containing L-[ϵ-15N]Lys using 15N cross-polarization magic-angle spinning NMR.
Quantified 69 peptidoglycan muropeptide ions from daptomycin-susceptible Enterococcus faecalis using liquid chromatography-mass spectrometry to evaluate structural modifications.
Daptomycin-treated S. aureus showed reduced lysyl-ε-amide intensity reflecting cell wall thinning without accumulation of the cytoplasmic precursor Park's nucleotide, indicating transglycosylation was not inhibited.
E. faecalis exhibited significant reductions in peptidoglycan crosslinking, decreased stem modifications by carboxypeptidases, and increased lytic transglycosylase activity, exposing immature walls to autolysin-driven degradation.

Cite This Study

Rimal et al. (2023) studied this question.

synapsesocial.com/papers/6a7d147ea3b6fd5035d5a126 https://doi.org/10.1038/s41598-023-39486-8

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Implication

In vitro analysis demonstrates impaired peptidoglycan crosslinking in Enterococcus faecalis under daptomycin, indicating reduced maturation triggers autolytic wall degradation.

The effects of daptomycin on cell wall biosynthesis in Enterococcal faecalis

Discussion

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Implication

Key Points

Cite This Study

The effects of daptomycin on cell wall biosynthesis in Enterococcal faecalis

Discussion

Member takes

Implication

Key Points

Cite This Study

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