What question did this study set out to answer?

To identify novel imprinted genes displaying monoallelic expression in the human placenta using high-throughput genotyping microarrays.

August 16, 2012EpigeneticsOpen Access

A genome-wide approach reveals novel imprinted genes expressed in the human placenta

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Genomic screening uncovers seven novel imprinted genes in human placenta, suggesting broader species-specific regulation than previously recognized.

To identify novel imprinted genes displaying monoallelic expression in the human placenta using high-throughput genotyping microarrays.
Adapted genotyping microarrays to contrast genotypes between cDNA derived from mRNA and genomic DNA in human placental tissue.
Screened and filtered candidate monoallelic single nucleotide polymorphisms to identify uncharacterized imprinted loci.
Generated 1,082 candidate monoallelic SNPs across more than 100 candidate genes, validating the approach against established imprinted genes including IPW, GRB10, INPP5F, and ZNF597.
Identified seven novel human imprinted genes (ZFAT, ZFAT-AS1, GLIS3, NTM, MAGI2, ZC3H12C, and LIN28B), four of which encode zinc finger transcription factors.
Determined that ZFAT demonstrates placental-specific paternal expression in endothelial cells and syncytiotrophoblasts and is downregulated in complicated pregnancies.

Barbaux et al. (2012) studied this question.

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Genomic screening uncovers seven novel imprinted genes in human placenta, suggesting broader species-specific regulation than previously recognized.