What question did this study set out to answer?

To evaluate FNDC5 gene expression in human skeletal muscle and adipose tissue alongside circulating irisin levels across varying degrees of obesity, insulin sensitivity, and type 2 diabetes.

February 23, 2013The Journal of Clinical Endocrinology & Metabolism

Irisin Is Expressed and Produced by Human Muscle and Adipose Tissue in Association With Obesity and Insulin Resistance

View Full Paper

Ask AI

Bookmark

Authors

JMJosé María Moreno‐NavarreteInstitut d'Investigació Biomèdica de Girona FOFrancisco OrtegaUniversitat Autònoma de Barcelona MSMarta SerranoHospital Universitario Miguel Servet

Discussion

Member takes

Overview

Observational cohort study finds decreased irisin levels and muscle-fat FNDC5 expression in obesity and diabetes, suggesting a loss of protective brown-like adipose characteristics.

Key Points

To evaluate FNDC5 gene expression in human skeletal muscle and adipose tissue alongside circulating irisin levels across varying degrees of obesity, insulin sensitivity, and type 2 diabetes.
Analyzed adipose FNDC5 expression and serum irisin by ELISA in two human cohorts (n = 125 and n = 76), including muscle biopsies from a subcohort of 34 participants.
Assessed irisin secretion in vitro using cultured human preadipocytes, mature adipocytes, and 3T3-L1 cells with induced browning via Rb1 gene knockdown.
Subcutaneous and visceral adipose tissue FNDC5 gene expression decreased significantly with obesity, positively correlating with brown fat and mitochondrial genes, and negatively correlating with LEP, TNFα, and FSP27.
Muscle expressed FNDC5 at 200-fold higher levels than fat, but its expression declined significantly in obese participants with type 2 diabetes.
Circulating irisin levels in men were negatively associated with obesity and insulin resistance, whereas in vitro cell browning triggered increased irisin secretion.

Cite This Study

Moreno‐Navarrete et al. (2013) studied this question.

synapsesocial.com/papers/6a7d243b00c5c9efbcaa0d87 https://doi.org/10.1210/jc.2012-2749

View Full Paper

Ask AI

Bookmark

February 23, 2013The Journal of Clinical Endocrinology & Metabolism

Irisin Is Expressed and Produced by Human Muscle and Adipose Tissue in Association With Obesity and Insulin Resistance

View Full Paper

Ask AI

Bookmark

Authors

JMJosé María Moreno‐NavarreteInstitut d'Investigació Biomèdica de Girona FOFrancisco OrtegaUniversitat Autònoma de Barcelona MSMarta SerranoHospital Universitario Miguel Servet

Discussion

Member takes

Overview

Observational cohort study finds decreased irisin levels and muscle-fat FNDC5 expression in obesity and diabetes, suggesting a loss of protective brown-like adipose characteristics.

Key Points

To evaluate FNDC5 gene expression in human skeletal muscle and adipose tissue alongside circulating irisin levels across varying degrees of obesity, insulin sensitivity, and type 2 diabetes.
Analyzed adipose FNDC5 expression and serum irisin by ELISA in two human cohorts (n = 125 and n = 76), including muscle biopsies from a subcohort of 34 participants.
Assessed irisin secretion in vitro using cultured human preadipocytes, mature adipocytes, and 3T3-L1 cells with induced browning via Rb1 gene knockdown.
Subcutaneous and visceral adipose tissue FNDC5 gene expression decreased significantly with obesity, positively correlating with brown fat and mitochondrial genes, and negatively correlating with LEP, TNFα, and FSP27.
Muscle expressed FNDC5 at 200-fold higher levels than fat, but its expression declined significantly in obese participants with type 2 diabetes.
Circulating irisin levels in men were negatively associated with obesity and insulin resistance, whereas in vitro cell browning triggered increased irisin secretion.

Cite This Study

Moreno‐Navarrete et al. (2013) studied this question.

synapsesocial.com/papers/6a7d243b00c5c9efbcaa0d87 https://doi.org/10.1210/jc.2012-2749

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark