Hydrogen sulfide attenuates doxorubicin-induced cardiotoxicity by inhibition of the p38 MAPK pathway in H9c2 cells

H9c2 cardiomyoblasts

Pretreatment with 400 µM sodium hydrogen sulfide (NaHS), 1,000 µM N-acetyl-L-cysteine (NAC), or 3 µM SB203580 prior to exposure to 5 µM doxorubicin (DOX)

Exposure to 5 µM doxorubicin (DOX) alone

Cardiomyocyte injuries including cytotoxicity, apoptosis, reactive oxygen species (ROS) generation, and dissipation of mitochondrial membrane potential (MMP)surrogate