What question did this study set out to answer?

To determine whether mitogen-activated protein (MAP) kinase activation functions downstream of high density lipoprotein (HDL)-induced protein kinase C stimulation in human skin fibroblasts.

September 1, 1997Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

High Density Lipoproteins Stimulate Mitogen-Activated Protein Kinases in Human Skin Fibroblasts

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Authors

MDMark A. DeegCompugen (Israel)RBRosario F. BowenRichard L. Roudebush VA Medical Center JOJohn F. OramRush University Medical Center

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Implication

In vitro study reveals HDL activates mitogen-activated protein kinases in human skin fibroblasts, indicating signaling independent of apolipoprotein-mediated cholesterol efflux.

Key Points

To determine whether mitogen-activated protein (MAP) kinase activation functions downstream of high density lipoprotein (HDL)-induced protein kinase C stimulation in human skin fibroblasts.
Measured kinase activation in human skin fibroblasts treated with HDL (50 µg protein/mL) using an in-gel kinase assay incorporating myelin basic protein.
Assessed dependency on protein kinase C (PKC) using PKC downregulation and specific inhibitors, while also evaluating effects of free apolipoproteins (A-I and A-II) and HDL modified with trypsin or tetranitromethane.
HDL stimulated multiple kinases (42, 50, 52, 58, and 60 kDa) maximally within 5 to 10 minutes for at least 60 minutes, with the 42-kDa MAP kinase (ERK1/ERK2) showing over a 2-fold activation.
Downregulation of PKC and chemical PKC inhibitors reduced HDL-induced MAP kinase activation by 40% to 50%.
Free apolipoproteins A-I and A-II (10 µg/mL) showed no significant activation of MAP kinase, and modifying HDL with trypsin or tetranitromethane did not alter kinase activation.

Cite This Study

Deeg et al. (1997) studied this question.

synapsesocial.com/papers/6a7d39f4e9ddcd9870aa1479 https://doi.org/10.1161/01.atv.17.9.1667

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Implication

In vitro study reveals HDL activates mitogen-activated protein kinases in human skin fibroblasts, indicating signaling independent of apolipoprotein-mediated cholesterol efflux.

High Density Lipoproteins Stimulate Mitogen-Activated Protein Kinases in Human Skin Fibroblasts

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Implication

Key Points

Cite This Study

High Density Lipoproteins Stimulate Mitogen-Activated Protein Kinases in Human Skin Fibroblasts

Discussion

Member takes

Implication

Key Points

Cite This Study

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