What question did this study set out to answer?

To determine whether plasma high-density lipoproteins stimulate the release of endothelin-1 in cultured human renal proximal tubular cells.

November 1, 1994Kidney InternationalOpen Access

Human high density lipoproteins stimulate endothelin-1 release by cultured human renal proximal tubular cells

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Why the study?

Does human HDL stimulate endothelin-1 release in cultured human renal proximal tubular cells?

Population

Cultured human renal proximal tubular cells

Comparison

Human high density lipoproteins vs Control, albumin preincubation, or modified HDL

Design

Preclinical

Follow-up

up to 12 hours

Authors

AOAlbert OngUniversity of Sheffield TJT.P. JowettUniversity College London JOJames S. OwenThe Royal Free Hospital

Discussion

Member takes

Implication

May implicate HDL in ET-1-mediated tubular injury; hypothesis-generating for nephrotic renal progression pending in vivo data.

Key Points

To determine whether plasma high-density lipoproteins stimulate the release of endothelin-1 in cultured human renal proximal tubular cells.
Exposed cultured human renal proximal tubular cells to human HDL subclasses (HDL2 and HDL3) across subconfluent and confluent states.
Tested lipid-depleted HDL3, albumin preincubation, and chemically modified HDL3 (including tetranitromethane modification and apoA-II substitution) across varying exposure durations.
Human HDL stimulated ET-1 secretion up to fourfold in a dose- and time-dependent manner, with greater effects in subconfluent cultures and similar potency between HDL2 and HDL3.
Partially and fully delipidated HDL3 reproduced the stimulatory effect, whereas replacing apolipoprotein A-I with apolipoprotein A-II reduced potency.
A 1-hour exposure to HDL3 was sufficient to trigger ET-1 release for the subsequent 12 hours, with peak activation occurring after 6 hours.

Structured PICO

Does human HDL stimulate endothelin-1 release in cultured human renal proximal tubular cells?

Population

Cultured human renal proximal tubular cells

Intervention

Human high density lipoproteins (HDL, HDL2, HDL3, and modified HDL3)

Comparator

Control (no HDL), albumin preincubation, or modified HDL

Outcome

Endothelin-1 (ET-1) secretionsurrogate

Human HDL stimulates endothelin-1 secretion in cultured human renal proximal tubular cells, suggesting a potential mechanism for progressive renal disease in nephrotic states.

Cite This Study

Ong et al. (1994) studied this question.

synapsesocial.com/papers/6a7d39f4e9ddcd9870aa147f https://doi.org/10.1038/ki.1994.400

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Human high density lipoproteins stimulate endothelin-1 release by cultured human renal proximal tubular cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Human high density lipoproteins stimulate endothelin-1 release by cultured human renal proximal tubular cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider