What question did this study set out to answer?

To characterize the resistance patterns and underlying beta-lactamase mechanisms among clinical isolates of imipenem-resistant Pseudomonas aeruginosa.

January 1, 1996Kitakantou igaku/Kitakantō IgakuOpen Access

Imipenem-Resistant Pseudomonas Aeruginosa

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Authors

MWMasato WatanabeTokyo National Hospital

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Implication

Microbiological study reveals transferable plasmid-mediated beta-lactamase activity in imipenem-resistant Pseudomonas aeruginosa isolates, suggesting broad resistance propagation.

Key Points

To characterize the resistance patterns and underlying beta-lactamase mechanisms among clinical isolates of imipenem-resistant Pseudomonas aeruginosa.
Categorized 70 imipenem-resistant clinical isolates of Pseudomonas aeruginosa into three groups based on minimum inhibitory concentrations against imipenem and ceftazidime.
Assayed baseline and induced beta-lactamase activity using cephaloridine and tested conjugative plasmid transfer into Pseudomonas aeruginosa and Escherichia coli.
Purified the beta-lactamase from the highly resistant strain GN17203 to determine molecular mass, isoelectric point, substrate hydrolysis, and inhibitor profiles.
Strains separated into Group A (20 strains; imipenem-resistant at 12.5 μg/ml, ceftazidime-sensitive at ≤1.5 μg/ml), Group B (49 strains; resistant to 12.5–25 μg/ml imipenem and 12.5–100 μg/ml ceftazidime), and Group C (1 strain; resistant to 25 μg/ml imipenem and ≥200 μg/ml ceftazidime).
Group C strain GN17203 harbored an imipenem-hydrolyzing beta-lactamase encoded on a 31-MDa IncP-9 plasmid (pMS350) conferring resistance to beta-lactams, gentamicin, and sulfonamides, transferable to P. aeruginosa but not E. coli.
The purified Group C enzyme exhibited an estimated molecular weight of 28,000, an isoelectric point of 9.0, broad hydrolysis across penicillins and cephalosporins, and inhibition by EDTA and heavy metals, but not clavulanic acid or sulbactam.

Cite This Study

Masato Watanabe (1996) studied this question.

synapsesocial.com/papers/6a7d412797c2c6e4659659e9 https://doi.org/10.2974/kmj1951.46.457

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Implication

Microbiological study reveals transferable plasmid-mediated beta-lactamase activity in imipenem-resistant Pseudomonas aeruginosa isolates, suggesting broad resistance propagation.

Imipenem-Resistant Pseudomonas Aeruginosa

Discussion

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Implication

Key Points

Cite This Study

Imipenem-Resistant Pseudomonas Aeruginosa

Discussion

Member takes

Implication

Key Points

Cite This Study

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