What question did this study set out to answer?

To determine how varying molar ratios of phospholipids and cholesterol in aqueous dispersions influence solvent extractability, ion sensitivity, enzymatic susceptibility, and sterol exchange with biological membranes.

May 1, 1968European Journal of BiochemistryOpen Access

Properties of Aqueous Dispersions of Phospholipid and Cholesterol

View Full Paper

Ask AI

Bookmark

Authors

KBK. Richard BruckdorferSt George's, University of London PEPeter A. EdwardsUniversity of California, Los Angeles CGC. GREEN

Discussion

Member takes

Implication

In vitro study reveals altered lipid extractability and exchange in phospholipid-cholesterol dispersions, suggesting structural rules that mimic lipoprotein and membrane dynamics.

Key Points

To determine how varying molar ratios of phospholipids and cholesterol in aqueous dispersions influence solvent extractability, ion sensitivity, enzymatic susceptibility, and sterol exchange with biological membranes.
Formulated aqueous dispersions containing phosphatidylcholine, phosphatidylethanolamine, or phosphatidic acid, alone or combined with cholesterol at defined molar ratios.
Tested lipid extraction into diethyl ether or chloroform in the presence of salts (NaCl, CaCl2) or polar organic solutes (urea, dimethylsulphoxide).
Evaluated sterol exchange with rat erythrocyte ghosts and susceptibility to phospholipase D degradation.
Nearly all cholesterol and approximately 60% of phosphatidylcholine were extractable from equimolar dispersions, whereas 2:1 phosphatidylcholine-to-cholesterol dispersions and other phospholipid mixtures resisted extraction.
Inorganic salts (NaCl, CaCl2) significantly increased lipid extraction from phosphatidylcholine and mixed dispersions, whereas urea and dimethylsulphoxide completely prevented it.
Equimolar dispersions exchanged sterols with rat erythrocytes, lower-cholesterol dispersions extracted up to 60% of membrane cholesterol, and mixed dispersions permitted phospholipase D attack without requiring ether.

Cite This Study

Bruckdorfer et al. (1968) studied this question.

synapsesocial.com/papers/6a7d543f1ec2a9317b425b2f https://doi.org/10.1111/j.1432-1033.1968.tb00241.x

View Full Paper

Ask AI

Bookmark

Implication

In vitro study reveals altered lipid extractability and exchange in phospholipid-cholesterol dispersions, suggesting structural rules that mimic lipoprotein and membrane dynamics.

Properties of Aqueous Dispersions of Phospholipid and Cholesterol

Discussion

Member takes

Implication

Key Points

Cite This Study

Properties of Aqueous Dispersions of Phospholipid and Cholesterol

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider