Haemolysis and myocardial and neural injury after monopolar pulsed field ablation using a novel lattice-tip catheter to treat atrial fibrillation

Figures

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  Figure 1. Temporal dynamics of (A) LDH (U/L), (B) total bilirubin (mg/dL), (C) haptoglobin (mg/dL) and (D) creatinine (mg/dL) from baseline (T0) compared to Day 1 (T1) post-PVI (AfferaTM, n = 40). ns, P > 0.05; ****, P ≤ 0.0001.

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  Figure 2. Temporal dynamics of haemolysis parameters, baseline (T0) compared to Day 1 (T1) post-PVI using different PFA platforms. Analysis of haemolysis parameters following PVI revealed a significant increase in bilirubin levels and a significant decrease in haptoglobin across all three PFA modalities. LDH levels increased significantly in the AfferaTM group, whereas no significant change was observed in PFA-P and PFA-F. ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P ≤ 0.0001.

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  Figure 3. Comparison of haemolysis parameters, (A) Δhaptoglobin (mg/dL), (B) ΔLDH (U/L), and (C) Δtotal bilirubin (mg/dL), and myocardial injury parameters, (D) Δtroponin (pg/dL), (E) ΔCK (U/L), and (F) ΔCK-MB (U/L) post-PVI using different PFA platforms. Comparison of haemolysis and myocardial injury parameters between all three modalities post-PVI compared to baseline showed a significantly higher (A) Δhaptoglobin decrease in PFA-F compared to AfferaTM and no significant difference in (B) ΔLDH and (C) Δbilirubin between all three PFA modalities. Myocardial injury markers (D) Δtroponin and (F) ΔCK-MB were comparable between all three groups. (E) ΔCK was significantly higher in AfferaTM compared to PFA-F. ns, P > 0.05; **, P < 0.01; ***, P < 0.001.

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  Figure 4. Temporal dynamics of S100 baseline (T0) compared to Day 1 (T1) post-ablation and ΔS100 (ug/L). Analysis of temporal dynamics revealed no significant change in S100 levels in the AfferaTM group, whereas a significant post-procedural increase was observed in both PFA-P and PFA-F compared to baseline. Comparison across all three PFA modalities demonstrated a significantly greater ΔS100 increase in PFA-F compared to AfferaTM. ns, P > 0.05; *, P < 0.05; **, P < 0.01.