Extraction of lipids from cottonseed tissue: VI. Ultrastructural morphology of isolated pigment glands

Cottonseed pigment glands, isolated from the underflow fraction of the liquid cyclone process, were examined with an electron microscope. The glands were circumscribed by a layer of tangentially flattened cells. Subjacent to the flattened cells were partially lysed cells containing recognizable remnants of cell walls. Within the lumens or matrices of the glands were myriads of pigment spherules measuring 0.1–1.5 µ in diameter. These spherules remained within the glands, even though gland walls were ruptured mechanically. Since aggregates of pigment spherules devoid of gland walls were observed in the gland‐rich fraction, it was concluded that maintenance of intact pigment glands was not a requisite for successful separation of gossypol from other cottonseed components with the liquid cyclone process. Avoiding dispersion of aggregated spherules from the glandular matrix was probably just as important as maintaining intact gland walls during mechanical separation of gossypol from other cottonseed components.