Liver DNA methylation of FADS2 associates with FADS2 genotypex

Figures

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  Fig. 1. Association between estimated delta-6 desaturase activity in serum and liver and DNA methylation of CpG sites in FADS2. A Spearman’s correlation was run to assess the relationship between methylation levels and delta-6 desaturase activity (n = 49 for serum and n = 19 for liver). Data is presented as Spearman correlation coefficient, and associations with a nominal p value < 0.05, which remained significant after correction for multiple testing using the Benjamini-Hochberg procedure with FDR 0.25, are indicated by boxes around the correlation coefficient. Positive correlations indicated with red and negative correlations with blue. Activity of delta-6 desaturase was estimated by a ratio of 18:3 n-6/18:2 n-6 in cholesteryl esters (CE) and triglycerides (TG)

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  Fig. 2. FADS2 DNA methylation associates with hepatic mRNA expression of FADS2. Scatterplots demonstrating correlations between methylation levels of cg06781209 (a) and cg07999042 (b) and the mRNA expression of FADS2 in liver. Spearman correlation coefficient was used for correlation analyses, and the statistical significance was corrected for multiple comparisons using the Benjamini–Hochberg procedure with a false discovery rate (FDR) of 0.25. Nominal p values are presented

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  Fig. 3. FADS2 DNA methylation levels according to groups based on FADS2 genotype. Panels (a) and (b) show DNA methylation levels in cg06781209 and cg07999042 according to FADS2 genotype (n = 88). Statistical significance was calculated with one-way ANOVA or a one-way Welch ANOVA with Bonferroni post-hoc test and nominal p values are presented