Hormone-dependent Phosphorylation of Ribosomal and Plasma Membrane Proteins in Mouse Mammary Gland in Vitro

Chemicals-Adenosine3') 5'-monophospha te, pancreatic DNase, colchicine, 0-phosphoserine, and 0-l)hosphothrconilIc were obtained from Calbiochem.Calf thymus histones, pancreatic RNase (five times crystallized), and crude collagenase were purchased from Worthington.cGMP, cUMP, and cCMP were products of Boehringer Mannheim, and [Y-~~P]-ATP (31 to 34 Ci per mmole) and inorganic [3'P]phosphate (carrier-free)were from International Chemical and Nuclear Corporation.[8-3H]Adenosine 3',5'-monophosphate (16.3 Ci per mmole) was obtained from Schwarz BioResearch.Cycloheximide was a product of Nutritional Biochemicals, density gradient grade sucrose (ribonuclease-free) was from Mann, and actinomycin D was obtained from AIerck.DEAE-cellulose and calcium phosphate gel, ATP, and bovine serum albumin were purchased from Sigma.Hydroxylapatite (Bio-Gel HT)