What question did this study set out to answer?

To determine how the enzyme Tdp1 and associated genetic pathways repair toxic topoisomerase I covalent complexes and resulting double-strand breaks in Saccharomyces cerevisiae.

August 1, 2001Genes to CellsOpen Access

Pathways for repair of topoisomerase I covalent complexes in Saccharomyces cerevisiae

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To determine how the enzyme Tdp1 and associated genetic pathways repair toxic topoisomerase I covalent complexes and resulting double-strand breaks in Saccharomyces cerevisiae.
Measured reaction kinetics and catalytic efficiency of yeast Tdp1 using model substrates with scissile bonds placed internally or at structural termini.
Assessed survival of yeast carrying wild-type or mutant topoisomerase I alongside single and double gene deletions in TDP1, RAD52, and RAD9 following toxic topoisomerase expression or camptothecin exposure.
Tdp1 showed relatively poor catalytic efficiency when the target bond was located in the middle of a DNA duplex, but exhibited markedly higher efficiency when situated at terminal ends.
Inactivation of TDP1 significantly reduced cell survival following induction of toxic topoisomerase I, placing TDP1 in the same epistasis group as RAD52 but in a different epistasis group from RAD9.
In rad9-deficient cells, TDP1 deletion reduced survival following camptothecin exposure specifically, while having no effect on survival against agents targeting other cellular pathways.

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Implication

Genetic and biochemical analysis reveals Tdp1-mediated repair of topoisomerase I lesions in yeast, indicating distinct double-strand break processing pathways.

Cite This Study

Pouliot et al. (2001) studied this question.

synapsesocial.com/papers/6a7d75542c71273e4183edfe https://doi.org/10.1046/j.1365-2443.2001.00452.x

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Implication

Genetic and biochemical analysis reveals Tdp1-mediated repair of topoisomerase I lesions in yeast, indicating distinct double-strand break processing pathways.

Pathways for repair of topoisomerase I covalent complexes in Saccharomyces cerevisiae

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Discussion

Implication

Cite This Study

Pathways for repair of topoisomerase I covalent complexes in Saccharomyces cerevisiae

Key Points

Discussion

Implication

Cite This Study

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