FEA-Guided Design and Experimental Validation of ZnO-Based Surface Acoustic Wave Biosensor with Au Sensing Layer for Label-Free EGFR L858R Mutation Detection
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Overview
Randomized trial demonstrates successful detection of EGFR mutation in biosensor, indicating potential clinical application.
Key Points
The aim is to develop and validate a ZnO-based surface acoustic wave biosensor for detecting the EGFR L858R mutation.
Fabricated ZnO-based devices on ZnO/Si and ZnO/SiO2/Si substrates using RF magnetron sputtering and UV lithography.
Conducted finite element analysis (2D and 3D) to analyze acoustic responses and verify experimental results.
Functionalized devices with a thiolated ssDNA probe for targeted detection of the EGFR L858R mutation.
ZnO/SiO2/Si device resonated at 145 MHz and 234 MHz for Rayleigh and Sezawa modes respectively, matching simulation.
Achieved a linear detection range of 0.1 to 0.6 µM and a limit of detection (LOD) of 0.09 µM for the EGFR mutation.
Signal transmission improved by approximately 2 dB with the incorporation of the Au sensing layer.