Mutagenesis-induced bacterial wilt resistance in bell pepper and multi-year validation of resistance by in vivo, ex vivo, serological, and molecular approaches

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This study aims to develop stable bacterial wilt resistance in bell pepper through induced mutagenesis.
Screened progenies under wilt-sick field conditions to identify resistant lines.
Validated resistance using in vivo field screening, ex vivo seedling assays, and serological confirmation with DAS-ELISA.
Performed molecular validation with SSR marker CAMS451 across multiple generations and cropping years.
Identified 22 putative resistant lines, with 16 showing stable resistance across generations and environments.
Six mutants outperformed the parent cultivar in yield and nutritional quality, with specific EMS and gamma radiation treatments noted.
SSR marker analysis confirmed the association of resistance-linked alleles with phenotypic resistance.

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Demonstrates bacterial wilt resistance in bell pepper, suggesting potential for sustainable breeding programs.

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A 2026 study studied this question.

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