What question did this study set out to answer?

Determine whether multidimensional flow cytometry can accurately differentiate leukemic from normal regenerating blasts to detect residual disease after chemotherapy and bone marrow transplantation.

July 1, 1998American Journal of Clinical PathologyOpen Access

Multidimensional Flow Cytometry of Marrow Can Differentiate Leukemic From Normal Lymphoblasts and Myeloblasts After Chemotherapy and Bone Marrow Transplantation

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Authors

DWDenise A. WellsChromoLogic (United States)GSGeorge E. SaleUniversity of Washington HSHoward M. ShulmanAmerican Cancer Society

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Implication

Observational cohort study demonstrates multidimensional flow cytometry predicts relapse in acute lymphoblastic leukemia, highlighting its role in guiding early intervention.

Key Points

Determine whether multidimensional flow cytometry can accurately differentiate leukemic from normal regenerating blasts to detect residual disease after chemotherapy and bone marrow transplantation.
Analyzed serial bone marrow aspirates from patients with acute lymphoblastic leukemia treated with chemotherapy, bone marrow transplantation (BMT), or both, using multidimensional flow cytometry with a 0.3% detection threshold.
Correlated flow cytometry findings with morphologic evaluation in 118 specimens and cytogenetic analyses before (N=62) and after (N=52) BMT.
Flow cytometry results matched morphology in 100 of 118 specimens, with flow-positive/morphology-negative samples verified by positive cytogenetics or subsequent relapse.
Patients with leukemic lymphoblasts identified by flow cytometry immediately prior to BMT had a 3.28-fold higher likelihood of post-transplant relapse compared with flow-negative patients.
All post-BMT patients with flow-detected leukemic lymphoblasts developed overt clinical relapse within 42 days of testing.

Cite This Study

Wells et al. (1998) studied this question.

synapsesocial.com/papers/6a7d76649022f402a7278dee https://doi.org/10.1093/ajcp/110.1.84

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Implication

Observational cohort study demonstrates multidimensional flow cytometry predicts relapse in acute lymphoblastic leukemia, highlighting its role in guiding early intervention.

Multidimensional Flow Cytometry of Marrow Can Differentiate Leukemic From Normal Lymphoblasts and Myeloblasts After Chemotherapy and Bone Marrow Transplantation

Discussion

Member takes

Implication

Key Points

Cite This Study

Multidimensional Flow Cytometry of Marrow Can Differentiate Leukemic From Normal Lymphoblasts and Myeloblasts After Chemotherapy and Bone Marrow Transplantation

Discussion

Member takes

Implication

Key Points

Cite This Study

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