What question did this study set out to answer?

To profile the hydrolysis rates of αS1-casein to αS1-I-casein during cheese ripening and understand the proteolytic behavior of different chymosin variants.

August 13, 2026Rapid Communications in Mass SpectrometryOpen Access

Profiling Hydrolysis Rates of αS1‐Casein to αS1‐I‐Casein During Cheese Ripening by Intact‐Protein Liquid Chromatography–High Resolution Mass Spectrometry

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Authors

SDSebastiaan DolmanFirmenich (Switzerland)MEMarjolein van der EijkFirmenich (Switzerland)LBLoes BeversFirmenich (Switzerland)

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Implication

Randomized trial analyzes cheese texture development via proteolytic activity of chymosins, suggesting enzyme influence on quality.

Key Points

To profile the hydrolysis rates of αS1-casein to αS1-I-casein during cheese ripening and understand the proteolytic behavior of different chymosin variants.
Employing intact-protein liquid chromatography-high-resolution mass spectrometry (LC-HRMS) to monitor hydrolysis rates during mozzarella cheese ripening.
Collecting cheese samples at multiple ripening stages using both bovine and camel chymosin variants.
Conducting protein extraction, separation, and quantification using optimized LC-HRMS protocols.
Bovine chymosins displayed significantly higher rates of αS1-casein hydrolysis to αS1-I-casein compared to camel chymosins throughout ripening.
Distinct phosphorylation variants were detected and quantified during cheese ripening.
Kinetic profiles showed consistent differences in proteolytic activity between coagulant types.

Cite This Study

Dolman et al. (2026) studied this question.

synapsesocial.com/papers/6a7d768a2b0e0cff3f63fef7 https://doi.org/10.1002/rcm.70153

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Profiling Hydrolysis Rates of αS1‐Casein to αS1‐I‐Casein During Cheese Ripening by Intact‐Protein Liquid Chromatography–High Resolution Mass Spectrometry

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Key Points

Cite This Study

Profiling Hydrolysis Rates of αS1‐Casein to αS1‐I‐Casein During Cheese Ripening by Intact‐Protein Liquid Chromatography–High Resolution Mass Spectrometry

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Implication

Key Points

Cite This Study

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