A strategy for decreasing prolamin content in rice endosperm through gene editing

Ziyan Shen; LI You-guang; Yuyan Huang; Tao Wang; Shijie Jian; Zihao Guan; Yuntong Feng; Xiangyuan Dang; Xinlan Shan; Shengyuan Sun; Min Guo; Zhi Hu; Yihao Yang; Changjie Yan

doi:10.1111/jipb.70371

August 11, 2026Journal of Integrative Plant Biology

A strategy for decreasing prolamin content in rice endosperm through gene editing

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Key Points

The aim is to decrease prolamin content in rice to enhance lysine levels and improve digestibility.
Utilized multiplex CRISPR editing to target prolamin genes.
Conducted phenotype-based screening to assess changes in protein content.
Identified and knocked out PROLM25 and PROLM26 genes.
Successfully reduced prolamin content leading to increased lysine levels.
Improved digestibility and overall taste of rice.
Knocking out PROLM25 and PROLM26 genes confirmed the desired effects.

Abstract

Multiplex CRISPR editing of prolamin genes and phenotype-based screening revealed that reducing prolamin content in rice can increase lysine content and digestibility, improve taste. PROLM25 and PROLM26 are key genes for reducing prolamin content, and knocking out these two genes can achieve the same effect.

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Cite This Study

Shen et al. (2026) studied this question.

synapsesocial.com/papers/6a7d768a2b0e0cff3f63ffc0 https://doi.org/10.1111/jipb.70371

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A strategy for decreasing prolamin content in rice endosperm through gene editing

Key Points

Abstract

Discussion

Cite This Study

A strategy for decreasing prolamin content in rice endosperm through gene editing

Key Points

Abstract

Discussion

Cite This Study

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