Endothelial ischemia-reperfusion injury in humans: association with age and habitual exercise

37 adults divided into four groups: young sedentary (n=10, 24 ± 2 yr), middle-aged sedentary (n=9, 48 ± 2 yr), young endurance-trained (n=9, 25 ± 1 yr), and middle-aged endurance-trained (n=9, 50 ± 2 yr).

20 minutes of continuous forearm occlusion to induce ischemia-reperfusion injury.

Baseline measurements (before occlusion) and comparisons across age and exercise groups.

Endothelial function assessed by brachial artery flow-mediated dilation (FMD) before and after occlusion.surrogate