What question did this study set out to answer?

To determine the inhibitory potency, selectivity, and mechanism of hexadecyl-N-methylpiperidinium (HMP) bromide against amyloid-beta (Aβ) fibril aggregation.

February 1, 1996Journal of Biological ChemistryOpen Access

Selective Inhibition of Aβ Fibril Formation

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Authors

SWStephen WoodOrygen Youth Health LMLauri MacKenzie BMBeverly E. MaleeffInformation Today (United States)

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Implication

In vitro study demonstrates selective inhibition of amyloid-beta fibril formation by HMP bromide, indicating a specific amphipathic binding site for anti-amyloid therapeutics.

Key Points

To determine the inhibitory potency, selectivity, and mechanism of hexadecyl-N-methylpiperidinium (HMP) bromide against amyloid-beta (Aβ) fibril aggregation.
Assayed in vitro Aβ aggregation and fibril formation in the presence of HMP bromide and structural analogs at pH 5.8 and pH 7.4.
Tested compound selectivity across other amyloidogenic polypeptides and measured effects on the folding stability of the beta-sheet-rich immunoglobulin REI domain.
HMP bromide selectively inhibited Aβ aggregation at pH 5.8 with an IC50 of 10 µM (approximately 1:1 stoichiometry) at concentrations well below its critical micelle concentration, without affecting REI stability or other amyloidogenic proteins.
Stoichiometric concentrations of HMP bromide inhibited Aβ fibril formation at pH 7.4, but lower concentrations were insufficient to block fibril growth seeded by preformed Aβ fibrils.

Cite This Study

Wood et al. (1996) studied this question.

synapsesocial.com/papers/6a7d8933d0946debe914fdc2 https://doi.org/10.1074/jbc.271.8.4086

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Implication

In vitro study demonstrates selective inhibition of amyloid-beta fibril formation by HMP bromide, indicating a specific amphipathic binding site for anti-amyloid therapeutics.

Selective Inhibition of Aβ Fibril Formation

Discussion

Member takes

Implication

Key Points

Cite This Study

Selective Inhibition of Aβ Fibril Formation

Discussion

Member takes

Implication

Key Points

Cite This Study

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