Complexation of Andrographolide, Mirabegron and Suvorexant and Bioactivity Study of the Complexes

Background: There are many ways to synthesize new drug molecules for more potent therapeutic efficacy with minimum side effects.The drugs may exhibit therapeutic effects independently or may interfere with each other.Method: In this study, new drug molecules were synthesized by drug-drug complexation way according to the calculated w/w ratio.The complexes were prepared by solid-mixing method using pestle and mortar by adding equal amount of drugs to each other, then warmed at 50 °C for 4 hours and left at room temperature for overnight to obtain new drug complexes.The new drug complexes were distinguished by Differential Scanning Calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR) with the biological activity.Results: The melting endothermic peaks of andrographolide-suvorexant, andrographolidemirabegron, and suvorexant-mirabegron complexes were found at 221.02 °C, 189.49°C and 115.78 °C, respectively which were different from the melting endotherms of andrographolide, suvorexant and mirabegron at 230.87 °C, 127.09 °C and 140.40 °C, respectively.The FTIR spectra of the formed complexes showed the similar characteristics of the standard drugs.The cytotoxic effect of the drug complexes on HeLa cell line were higher (10% less survived HeLa cell, i.e. > 90 % cell died) than the precursor drug molecules.Conclusion: Our research findings that three new drug compounds were synthesized by complexation way which could be due to interaction of andrographolide and suvorexant; andrographolide and mirabegron, and suvorexant and mirabegron.