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The active species of CO2, i.e. CO2 or HCO3-, utilized in the pyruvate carboxylase and phosphoenolypruvate carboxylase reaction have been determined with an assay predicated upon the fact that the hydration of CO2 is a kinetically slow and measurable reaction. The results of these assays in both cases are in reasonable agreement with those expected if HCO3- is assumed to be the functional species utilized by both enzymes.
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Cooper et al. (1971) studied this question.
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