The association between mRNA expression of resistin, TNF- α, IL-6, IL-8, and ER-α in peripheral blood mononuclear cells and breast cancer

Figures

• 

  Figure 1. The pattern of PCR products was amplified by GAPDH, Resistin, TNF-α, IL-6, IL-8, and ER-α specific primers. Lane M: 50 bp ladder (Fermentas, Germany), Lane 2: GAPDH product (194 bp); Lane 3: Nontemplate control (NTC); Lane 4: Resistin product (119 bp); Lane 5: NTC; Lane 6: TNF-α product (102 bp); Lane 7: NTC; Lane 8: IL-6 product (164 bp); Lane 9: NTC; lane 10; IL-8 product (68 bp); lane 11: NTC; Lane 12: ER-α product (85 bp); Lane 13: NTC.

• 

  Figure 2. The mRNA expression of resistin in PBMCs from breast cancer patients and controls. Fold change expression of resistin in breast cancer patients compared to those from healthy controls determined using a quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR). Fold change in mRNA level is shown under each bar. Error bars are standard errors of the means for resistin expression. Data are expressed as mean ± S.E.M. REST: Resistin.

• 

  Figure 3. The mRNA expression of IL-6 in PBMCs from breast cancer patients and controls. Fold change expression of IL-6 in breast cancer patients compared to those from healthy controls determined using qRT-PCR. Fold change in mRNA level is shown under each bar. Error bars are standard errors of the means for IL-6 expression. Data are expressed as mean ± S.E.M.

• 

  Figure 4. The mRNA expression of TNF-α in PBMCs from breast cancer patients and controls. Fold change expression of TNF-α in breast cancer patients compared to those from healthy controls determined using qRT-PCR. Fold change in mRNA level shown under each bar. Error bars are standard errors of the means for TNF-α expression. Data are expressed as mean ± S.E.M

• 

  Figure 5. The mRNA expression of IL-8 in PBMCs from breast cancer patients and controls. Fold change expression of IL-8 in breast cancer patients compared to those from healthy controls determined by qRT-PCR. Fold change in mRNA level is shown under each bar. Error bars are standard errors of the means for IL-8 expression. Data are expressed as mean ± S.E.M.

• 

  Figure 6. The mRNA expression of ER-α in PBMCs from breast cancer patients and controls. Fold change expression of ER-α in breast cancer patients compared to those from healthy controls determined using qRT-PCR. Fold change in mRNA level is shown under each bar. Error bars are standard errors of the means for ER-α expression. Data are expressed as mean ± S.E.M.