What question did this study set out to answer?

To evaluate differences in membrane protein abundance between virulent H37Rv and attenuated H37Ra strains of Mycobacterium tuberculosis to identify drivers of bacterial virulence.

January 24, 2011BMC MicrobiologyOpen Access

Comparison of membrane proteins of Mycobacterium tuberculosisH37Rv and H37Ra strains

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Authors

HMHiwa MålenUniversity of Bergen GSGustavo A. de SouzaUniversidade Federal do Rio Grande do Norte SPSharad PathakOslo University Hospital

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Implication

Comparative proteomic study reveals distinct membrane transport protein abundances between virulent and attenuated Mycobacterium tuberculosis strains, suggesting secretion systems mediate virulence.

Key Points

To evaluate differences in membrane protein abundance between virulent H37Rv and attenuated H37Ra strains of Mycobacterium tuberculosis to identify drivers of bacterial virulence.
Applied a label-free quantitative proteomic approach to compare relative protein abundance across the two closely related M. tuberculosis strains.
Profiled the bacterial proteomes to identify proteins exhibiting at least a 5-fold differential expression in membrane-associated fractions.
Identified over 1,700 total proteins, with most exhibiting equivalent abundance between H37Rv and H37Ra.
Observed 29 membrane-associated proteins with a ≥5-fold difference in relative abundance: 19 membrane- and lipoproteins were elevated in H37Rv, and 10 were elevated in H37Ra.
Identified the putative protein-export membrane protein SecF (Rv2586c) and three ABC-transporters (Rv0933, Rv1273c, Rv1819c) among the proteins enriched in the virulent H37Rv strain.

Cite This Study

Målen et al. (2011) studied this question.

synapsesocial.com/papers/6a7d9f23da6d5413e2cb4eec https://doi.org/10.1186/1471-2180-11-18

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Implication

Comparative proteomic study reveals distinct membrane transport protein abundances between virulent and attenuated Mycobacterium tuberculosis strains, suggesting secretion systems mediate virulence.

Comparison of membrane proteins of Mycobacterium tuberculosisH37Rv and H37Ra strains

Discussion

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Implication

Key Points

Cite This Study

Comparison of membrane proteins of Mycobacterium tuberculosisH37Rv and H37Ra strains

Discussion

Member takes

Implication

Key Points

Cite This Study

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