What question did this study set out to answer?

To determine the role of NLRP1 and NLRP3 inflammasomes during ischemic stroke and evaluate whether intravenous immunoglobulin (IVIg) can suppress their activity to protect neurons.

September 5, 2013Cell Death and DiseaseOpen Access

Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke

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Authors

DFDavid Y. FannNational University of Singapore SMSilvia ManzaneroQueensland University of Technology MGMathias GelderblomUniversität Hamburg

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Implication

Preclinical study demonstrates IVIg suppresses NLRP1 and NLRP3 inflammasome-mediated neurotoxicity in stroke models, suggesting a therapeutic strategy to reduce ischemic brain injury.

Key Points

To determine the role of NLRP1 and NLRP3 inflammasomes during ischemic stroke and evaluate whether intravenous immunoglobulin (IVIg) can suppress their activity to protect neurons.
Subjected primary cortical neurons to glucose deprivation, oxygen-glucose deprivation, or simulated ischemia-reperfusion in vitro.
Induced ischemic stroke in C57BL/6J mice via middle cerebral artery occlusion with reperfusion to measure brain damage, behavioral deficits, and inflammasome activation.
Quantified NLRP1 and NLRP3 inflammasome markers in postmortem human stroke brain samples and tested the protective effects of caspase-1 inhibition and IVIg.
NLRP1 and NLRP3 inflammasome protein levels, as well as downstream cytokines IL-1β and IL-18, were elevated following ischemia-like insults in primary neurons, mouse stroke models, and human stroke tissue.
Pharmacological inhibition of caspase-1 protected cultured cortical neurons and reduced brain cell damage in vivo.
IVIg treatment significantly reduced neuronal death in stroke models by suppressing NLRP1 and NLRP3 inflammasome activity.

Cite This Study

Fann et al. (2013) studied this question.

synapsesocial.com/papers/6a7da2ae7c193befc433994a https://doi.org/10.1038/cddis.2013.326

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Implication

Preclinical study demonstrates IVIg suppresses NLRP1 and NLRP3 inflammasome-mediated neurotoxicity in stroke models, suggesting a therapeutic strategy to reduce ischemic brain injury.

Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke

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Implication

Key Points

Cite This Study

Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke

Discussion

Member takes

Implication

Key Points

Cite This Study

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