What question did this study set out to answer?

To determine the role of the conserved oligopeptide permeases Opp and App in regulating sporulation initiation and disease pathogenesis in Clostridium difficile.

July 29, 2014Infection and ImmunityOpen Access

Conserved Oligopeptide Permeases Modulate Sporulation Initiation in Clostridium difficile

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To determine the role of the conserved oligopeptide permeases Opp and App in regulating sporulation initiation and disease pathogenesis in Clostridium difficile.
Inactivated the opp and app oligopeptide permease genes in C. difficile and assayed timing of early sporulation gene expression and spore production in vitro.
Assessed bacterial virulence and measured spore burdens shed in feces using an in vivo hamster model of C. difficile infection.
Inactivation of opp and app induced earlier expression of early sporulation genes and significantly increased spore formation in vitro.
Disrupting opp and app resulted in heightened virulence and increased recovery of spores from feces in infected hamsters.
Permeases were shown to indirectly repress sporulation, operating via the transcriptional regulator SinR and its antagonist SinI.

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Implication

Experimental study reveals that disrupting oligopeptide permeases accelerates Clostridium difficile sporulation and virulence in hamsters, indicating nutrient availability restrains transmission.

Cite This Study

Edwards et al. (2014) studied this question.

synapsesocial.com/papers/6a7da348050e98c0187d82a8 https://doi.org/10.1128/iai.02323-14

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Implication

Experimental study reveals that disrupting oligopeptide permeases accelerates Clostridium difficile sporulation and virulence in hamsters, indicating nutrient availability restrains transmission.

Conserved Oligopeptide Permeases Modulate Sporulation Initiation in Clostridium difficile

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Discussion

Implication

Cite This Study

Conserved Oligopeptide Permeases Modulate Sporulation Initiation in Clostridium difficile

Key Points

Discussion

Implication

Cite This Study

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