What question did this study set out to answer?

To determine the requirement and quantitative contribution of the PYR/PYL/RCAR receptor family to abscisic acid perception, stomatal control, and transcriptional responses in Arabidopsis.

June 1, 2012The Plant CellOpen Access

Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid

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To determine the requirement and quantitative contribution of the PYR/PYL/RCAR receptor family to abscisic acid perception, stomatal control, and transcriptional responses in Arabidopsis.
Generated and evaluated higher-order Arabidopsis thaliana mutants lacking multiple PYR/PYL receptors, including a sextuple mutant (pyr1 pyl1 pyl2 pyl4 pyl5 pyl8).
Assessed seed germination and vegetative growth under 100 μM abscisic acid, along with whole-rosette stomatal conductance (Gst) and leaf transpiration measurements.
Analyzed abscisic acid-induced SnRK2 kinase activation and downstream transcriptional responses.
The sextuple pyr/pyl mutant germinated and grew in 100 μM abscisic acid and exhibited higher stomatal conductance and transpiration than aba3-1 or snrk2.6 mutants.
Stomatal conductance increased stepwise across plants lacking three, four, five, and six PYR/PYL receptors, demonstrating quantitative control of stomatal aperture.
The sextuple mutant completely lacked abscisic acid-mediated SnRK2 activation and displayed severely impaired hormone-responsive gene expression.

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Implication

Genetic analysis reveals dose-dependent regulation of stomatal aperture by PYR/PYL receptors in Arabidopsis, indicating their central role in abscisic acid signaling.

Cite This Study

González‐Guzmán et al. (2012) studied this question.

synapsesocial.com/papers/6a7daafa10b6f5f3737d89cc https://doi.org/10.1105/tpc.112.098574

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Implication

Genetic analysis reveals dose-dependent regulation of stomatal aperture by PYR/PYL receptors in Arabidopsis, indicating their central role in abscisic acid signaling.

Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid

Key Points

Discussion

Implication

Cite This Study

Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid

Key Points

Discussion

Implication

Cite This Study

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