Why the study?
Does the addition of aprotinin affect the stability of plasma concentrations of C-terminal ANP in blood samples maintained at room temperature or 0°C?
Does the addition of aprotinin affect the stability of plasma concentrations of C-terminal ANP in blood samples maintained at room temperature or 0°C?
The addition of aprotinin is not necessary for maintaining the stability of C-terminal ANP in whole blood samples kept at room temperature or 0°C for up to 3 hours.
Aprotinin may be omitted for short-term C-terminal ANP sample stability; leaves open broader validation across conditions and assays.
In our -own investigations we examined the effect of delayed separation where the sam- ple, collected into polypropylene tubes con- taining EDTA, with and without the addition of aprotinin, was maintained as whole blood at room temperature and at 0°C for one to three hours.Plasma ANP levels were measured by radioimmunoassay following Sep-pak plasma extraction as previously described.2The results demonstrated that the addition of aprotinin made little difference to the plasma concentration of the C-terminal ANP (table).
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McDowell et al. (1997) studied this question.
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