Low periostin expression predicts poor survival in intestinal type gastric cancer patients

Figures

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  Figure 1. The process flow of each spot in the TMA. Abbreviations: AOD, average optical density; IHC, immunohistochemistry; IOD, integrated optical density; TMA, tissue microarray.

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  Figure 2. Periostin expression in GC and adjacent normal tissue. Notes: (A, D, G, and I) Periostin staining in epithelial cell-positive normal and tumor tissues. (B and E) Low expression of periostin in epithelial cell-negative normal and tumor tissues. (C, F, H, and J) High expression of periostin in epithelial cell-negative normal and tumor tissue. (K and L) Differential expression of cellular and stromal periostin between tumor and adjacent normal tissue. (M and N) Differential expression of cellular and stromal periostin between intestinal type and diffuse type GC. Abbreviations: AOD, average optical density; GC, gastric cancer.

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  Figure 3. Periostin epithelial cell negative and low expression in epithelial cell-negative tissue were associated with the poor survival of GC patients. Notes: (A) Different survivals between patients with periostin epithelial cell-negative or periostin epithelial cell-positive GC. (B) Different survivals between patients with stromal periostin low-expression or high-expression GC whose periostin was negative in epithelial cell tumors. Log-rank P-values are derived from Kaplan–Meier analysis with log-rank test. Abbreviation: GC, gastric cancer.

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  Figure 4. Different survivals between patients with different expression levels of periostin in GEO data sets, GSE14210, GSE15459, GSE22377, GSE29272, GSE51105, and GSE62254. Note: Log-rank P-values are derived from Kaplan–Meier analysis with log-rank test. Abbreviation: GEO, Gene Expression Omnibus.

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  Figure 5. The loss of periostin expression is associated with worse survival of intestinal type GC patients compared to that of diffuse type GC patients. Notes: (A and B) Different survivals between patients with intestinal type GC according to the cellular or stromal periostin expression. (C and D) Different survivals between patients with diffuse type GC according to the cellular or stromal periostin expression. Log-rank P-values are derived from Kaplan–Meier analysis with log-rank test. Abbreviation: GC, gastric cancer.

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  Figure 6. Expression of CD163 in different periostin expression tumor tissues. Notes: (A and B) Neither epithelial cellular periostin nor stromal periostin expression was associated with expression of CD163 in intestinal type GC. (C and D) Epithelial cellular periostin and stromal periostin had a positive area/total correlation with CD163 in diffuse type GC. Relative intensity of CD163 was represented by positive area/total area of the spot).