Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages

This paper describes a procedure for the separation of lecithins according to the number of ethylenic bonds in their fatty acid residues. The procedure uses a column of alkylated dextran (Sephadex LH-20) eluted with an organic solvent system, the unsaturated lipids being separated as their mercuric acetate addition compounds. The system is capable of resolving at least four species of lecithin, and the intact lecithin molecules can be recovered for further study. The chromatographic system has been tested with lecithin derived from dog lung, rat liver, and hen's egg.