What question did this study set out to answer?

To determine the evolutionary origin of the three-component 4-chlorobenzoate (4-CBA) dehalogenase enzyme complex from Pseudomonas sp. CBS-3 using amino acid sequence homology.

June 1, 1992Biochemistry

Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases

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Authors

PBPatricia C. BabbittQB3 GKGeorge L. KenyonRutgers, The State University of New Jersey BMBrian M. MartinHospital Universitario de Getafe

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Implication

Sequence homology analysis reveals evolutionary links between 4-chlorobenzoate dehalogenase and beta-oxidation enzymes in Pseudomonas, indicating recruitment from ancestral fatty acid pathways.

Key Points

To determine the evolutionary origin of the three-component 4-chlorobenzoate (4-CBA) dehalogenase enzyme complex from Pseudomonas sp. CBS-3 using amino acid sequence homology.
Deduced the complete nucleotide sequences for the three open reading frames (ORF1, ORF2, and ORF3) encoding 4-chlorobenzoate dehalogenase in Pseudomonas sp. CBS-3.
Performed comparative amino acid sequence alignments against known families of acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases.
ORF1 (30-kDa 4-CBA-CoA dehalogenase) exhibited strong sequence homology to enoyl-CoA hydratases involved in fatty acid beta-oxidation.
ORF2 (57-kDa 4-CBA:CoA ligase) demonstrated significant similarity to enzymes in beta-oxidation and antibiotic synthesis, sharing a highly conserved (T,S)(S,G)G(T,S)(T,E)G(L,X)PK(G,-) signature sequence.
ORF3 (16-kDa thioesterase) showed no detectable sequence homology to known proteins, though overall reaction chemistry supports evolution of the pathway from ancestral beta-oxidation systems.

Cite This Study

Babbitt et al. (1992) studied this question.

synapsesocial.com/papers/6a7dd3b6e5379bb2b27d8ec7 https://doi.org/10.1021/bi00139a024

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Implication

Sequence homology analysis reveals evolutionary links between 4-chlorobenzoate dehalogenase and beta-oxidation enzymes in Pseudomonas, indicating recruitment from ancestral fatty acid pathways.

Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases

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Cite This Study

Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases

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