November 10, 2011AJP Cell PhysiologyOpen Access

ANOs 3–7 in the anoctamin/Tmem16 Cl − channel family are intracellular proteins

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Population

HEK293, COS-7, CHO, and FRT cell lines, 22Rv1-ANO7 cell line, and human prostate tissue.

Comparison

Heterologous expression of anoctamins ANO3… vs Cells expressing GFP alone, ANO1, or ANO2.

Design

Preclinical

Authors

CDCharity DuranEmory University ZQZhiqiang QuXidian University AOAdeboye O. OsunkoyaEmory University

Discussion

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Implication

ANOs 3-7 should not be modeled as plasma membrane Ca2+-activated Cl- channels; leaves open their intracellular roles in Ca2+ handling.

Structured PICO

Population

HEK293, COS-7, CHO, and FRT cell lines, 22Rv1-ANO7 cell line, and human prostate tissue.

Intervention

Heterologous expression of anoctamins ANO3, ANO4, ANO5, ANO6, ANO7, ANO10, and chimeric constructs of ANO1 and ANO5/7.

Comparator

Cells expressing GFP alone, ANO1, or ANO2.

Outcome

Generation of Cl- currents activated by intracellular Ca2+ (measured by whole cell patch clamp) and subcellular localization (measured by confocal imaging).surrogate

Unlike ANO1 and ANO2, anoctamins 3-7 are predominantly intracellular proteins that do not function as plasma membrane calcium-activated chloride channels when heterologously expressed.

Limitations

Potential artifacts of overexpression in a heterologous system

Cite This Study

Duran et al. (2011) studied this question.

synapsesocial.com/papers/6a7dd722286d888403cdaeda https://doi.org/10.1152/ajpcell.00140.2011

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ANOs 3–7 in the anoctamin/Tmem16 Cl − channel family are intracellular proteins

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

ANOs 3–7 in the anoctamin/Tmem16 Cl − channel family are intracellular proteins

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider