What question did this study set out to answer?

To determine how cell type and stimulus shape genome-wide transcription factor binding and transcriptional output during the heat shock response.

December 15, 2022Open Access

Transcriptional Responses of Cancer Cells to Heat Shock-Inducing Stimuli Involve Amplification of Robust HSF1 Binding

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Authors

SDSayantani Ghosh DastidarIllumina (United States)BKBony De KumarYale Cancer Center BLBo LaucknerUniversity of North Dakota

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Implication

In vitro study reveals robust HSF1 binding driving stimulus-specific transcription in cancer cell lines, indicating direct engagement with inactive chromatin.

Key Points

To determine how cell type and stimulus shape genome-wide transcription factor binding and transcriptional output during the heat shock response.
Induced the heat shock response pathway in two distinct cell lines using two separate activating stimuli.
Mapped genome-wide binding profiles of the master regulator HSF1 and correlated them with nascent RNA synthesis and chromatin accessibility.
HSF1 binding patterns consistently maintained their genomic distribution across differing activation magnitudes, linking conserved binding sites to stimulus-specific transcriptional responses.
HSF1 activation induced a modest overall increase in chromatin accessibility that occurred predominantly at remote genomic regions and inactive chromatin rather than solely at established promoters and enhancers.

Cite This Study

Dastidar et al. (2022) studied this question.

synapsesocial.com/papers/6a7ddaebc2b228f3e18ca526 https://doi.org/10.1101/2022.12.14.519647

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Implication

In vitro study reveals robust HSF1 binding driving stimulus-specific transcription in cancer cell lines, indicating direct engagement with inactive chromatin.

Transcriptional Responses of Cancer Cells to Heat Shock-Inducing Stimuli Involve Amplification of Robust HSF1 Binding

Discussion

Member takes

Implication

Key Points

Cite This Study

Transcriptional Responses of Cancer Cells to Heat Shock-Inducing Stimuli Involve Amplification of Robust HSF1 Binding

Discussion

Member takes

Implication

Key Points

Cite This Study

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