What question did this study set out to answer?

To evaluate how scaffold topography and nanofiber alignment influence in vitro cellular adhesion and in vivo foreign body reactions in tissue engineering applications.

September 18, 2009Journal of Biomedical Materials Research Part AOpen Access

The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction

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Authors

HCHaoqing CaoZhengzhou University KMKevin J. McHughRice University SCSing Yian ChewNanyang Technological University

Discussion

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Implication

Preclinical study demonstrates reduced monocyte adhesion and thinner fibrous capsule formation around electrospun aligned nanofibers, suggesting improved biocompatibility for tissue engineering.

Key Points

To evaluate how scaffold topography and nanofiber alignment influence in vitro cellular adhesion and in vivo foreign body reactions in tissue engineering applications.
Fabricated random (313 ± 5 nm) and aligned (506 ± 24 nm) electrospun polycaprolactone (PCL) nanofibers, smooth PCL films, and RGD-coated glass controls.
Assessed primary human monocyte adhesion and morphology in vitro (N=5 donors) across days 0, 3, 7, and 10, and evaluated subcutaneous host responses in rats at weeks 1, 2, and 4.
Initial in vitro monocyte adhesion was significantly lower on aligned nanofiber scaffolds compared to random nanofiber scaffolds (p < 0.001).
Fibrous capsule thickness in vivo was 7.55 ± 0.54 µm for aligned fibers and 4.13 ± 0.31 µm for random fibers, both significantly thinner than solid film implants at 37.7 ± 0.25 µm (p < 0.001), with aligned fibers promoting cellular infiltration.

Cite This Study

Cao et al. (2009) studied this question.

synapsesocial.com/papers/6a7df656a76b9ebabb045cfb https://doi.org/10.1002/jbm.a.32609

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September 18, 2009Journal of Biomedical Materials Research Part AOpen Access

The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction

View Full Paper

Ask AI

Bookmark

Authors

HCHaoqing CaoZhengzhou University KMKevin J. McHughRice University SCSing Yian ChewNanyang Technological University

Discussion

Member takes

Implication

Preclinical study demonstrates reduced monocyte adhesion and thinner fibrous capsule formation around electrospun aligned nanofibers, suggesting improved biocompatibility for tissue engineering.

Key Points

To evaluate how scaffold topography and nanofiber alignment influence in vitro cellular adhesion and in vivo foreign body reactions in tissue engineering applications.
Fabricated random (313 ± 5 nm) and aligned (506 ± 24 nm) electrospun polycaprolactone (PCL) nanofibers, smooth PCL films, and RGD-coated glass controls.
Assessed primary human monocyte adhesion and morphology in vitro (N=5 donors) across days 0, 3, 7, and 10, and evaluated subcutaneous host responses in rats at weeks 1, 2, and 4.
Initial in vitro monocyte adhesion was significantly lower on aligned nanofiber scaffolds compared to random nanofiber scaffolds (p < 0.001).
Fibrous capsule thickness in vivo was 7.55 ± 0.54 µm for aligned fibers and 4.13 ± 0.31 µm for random fibers, both significantly thinner than solid film implants at 37.7 ± 0.25 µm (p < 0.001), with aligned fibers promoting cellular infiltration.

Cite This Study

Cao et al. (2009) studied this question.

synapsesocial.com/papers/6a7df656a76b9ebabb045cfb https://doi.org/10.1002/jbm.a.32609

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