What question did this study set out to answer?

Characterize the global transcriptional response and identify newly regulated genes in Bacillus subtilis following exposure to heat shock.

December 15, 2001Journal of BacteriologyOpen Access

Global Transcriptional Response of Bacillus subtilis to Heat Shock

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Authors

JHJohn D. HelmannNational Institutes of Health MWMing‐Fang WuChung Shan Medical University Hospital PKPhil A. KobelLa Roche College

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Implication

Microarray profiling reveals widespread regulon activation and metabolic shifts in heat-shocked Bacillus subtilis, highlighting broader coordination across general stress response pathways.

Key Points

Characterize the global transcriptional response and identify newly regulated genes in Bacillus subtilis following exposure to heat shock.
Monitored genome-wide transcriptional alterations over time using DNA microarray hybridization after heat shock exposure.
Analyzed promoter DNA sequences upstream of novel heat-induced genes to identify regulon membership and verified gene expression via quantitative reverse transcriptase PCR.
Observed rapid, transient induction of sigma(B)-dependent genes alongside slower-activating HrcA and CtsR heat shock regulons.
Identified approximately 70 putative new members of the sigma(B) regulon based on regulatory sequence analysis.
Discovered robust heat-induced transcriptional activation of arginine biosynthesis and transport operons outside known heat shock regulons.

Cite This Study

Helmann et al. (2001) studied this question.

synapsesocial.com/papers/6a7df67a95d2f4a0c85c9a02 https://doi.org/10.1128/jb.183.24.7318-7328.2001

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Implication

Microarray profiling reveals widespread regulon activation and metabolic shifts in heat-shocked Bacillus subtilis, highlighting broader coordination across general stress response pathways.

Global Transcriptional Response of Bacillus subtilis to Heat Shock

Discussion

Member takes

Implication

Key Points

Cite This Study

Global Transcriptional Response of Bacillus subtilis to Heat Shock

Discussion

Member takes

Implication

Key Points

Cite This Study

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