Why the study?
Does the KO1 mutation in adenoviral vectors ablate hepatic transduction in vivo?
Population
A549 cells, primary fibroblast cells, hepatocytes in vitro, and three different mouse strains in vivo
Comparison
Adenoviral vector with S408E-P409A mutation in… vs Unmodified adenoviral vectors
Design
Preclinical
Authors
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KO1 mutation fails to detarget hepatocytes in mice; leaves open whether additional capsid modifications can achieve fully detargeted adenoviral vectors.
Does the KO1 mutation in adenoviral vectors ablate hepatic transduction in vivo?
In vivo hepatic adenoviral gene transfer occurs independently of the coxsackievirus-adenovirus receptor, indicating that further viral capsid modifications are required to generate a fully detargeted vector.
Smith et al. (2002) studied this question.
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