What question did this study set out to answer?

To observe the initial structural lesions and cell wall-membrane alterations induced by penicillin in Bacillus megaterium under varying osmotic conditions.

August 1, 1965The Journal of Cell BiologyOpen Access

The Initial Structural Lesion of Penicillin Action in Bacillus Megaterium

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To observe the initial structural lesions and cell wall-membrane alterations induced by penicillin in Bacillus megaterium under varying osmotic conditions.
Cultured Bacillus megaterium cells in media with and without osmotic stabilization.
Tracked structural and morphological alterations across 10-minute and 20-minute intervals following penicillin administration.
Without osmotic support, cells developed membrane-wall distortion and cytoplasmic leakage by 20 minutes, which was rapidly followed by cell lysis.
With osmotic support, a localized transverse-septal lesion appeared by 10 minutes, generating a membrane pocket filled with unorganized fibrous wall mucopeptide.
The fibrous pocket progressively enlarged until cells either underwent lysis or converted into protoplasts.

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Implication

Experimental study demonstrates transverse-septal lesions and mucopeptide disruption in Bacillus megaterium, indicating localized cell wall failure under penicillin exposure.

Cite This Study

Fitz-James et al. (1965) studied this question.

synapsesocial.com/papers/6a7e04d7f980e836411885f7 https://doi.org/10.1083/jcb.26.2.657

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Implication

Experimental study demonstrates transverse-septal lesions and mucopeptide disruption in Bacillus megaterium, indicating localized cell wall failure under penicillin exposure.

The Initial Structural Lesion of Penicillin Action in Bacillus Megaterium

Key Points

Discussion

Implication

Cite This Study

The Initial Structural Lesion of Penicillin Action in Bacillus Megaterium

Key Points

Discussion

Implication

Cite This Study

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