Recent work has indicated that temperature can no longer be neglected as a column performance parameter in liquid chromatography. The work presented in this paper investigates the temperature dependence of the polar and non-polar forces which determine the distribution coefficient of a solute adsorbed on silicic acid. The effect of temperature on the solute retention indicates that if the technique is to achieve the same advanced stage as gas chromatography, the column temperature must be controlled. The retention volumes of several compounds have been examined over the temperature range −20°C to +70°C for a variety of mobile phases. The results have been discussed from the point of view of selectivity temperature control and temperature programming in liquid chromatography.
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R. J. Maggs (1969) studied this question.